Milli-Mark Anti-CD13-FITC

Analysis Note

ControlPBMCs

Application

Detect CD13 using this Milli-Mark Anti-CD13-FITC Antibody, clone WM-47 validated for use in FC.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD13 is a 150 kDa Type II integral membrane protein composed of 967 amino acids (Turni, 2002; Ashmun, 1995; Look, 1989). CD13 is identical in structure to aminopeptidase N, a membrane-bound zinc-binding metalloprotease (Turni, 2002). CD13 is expressed on the surface of committed granulocyte-monocyte progenitors (CFU-GM) and by cells of the granulocyte and monocyte lineages at all stages of differentiation (Turni, 2002; Ashmun, 1995; Look, 1989). The antigen is also expressed on a small proportion of large granular lymphocytes, but not on B or T cells (Ashmun, 1995; Look, 1989). Further, CD13 is expressed on some non-hematopoietic cells including fibroblasts, endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining the bile duct canaliculi (Turni, 2002; Ashmun, 1995; Look, 1989).
Anti-CD13, WM-47, was included in the Fourth and Fifth International Workshop and Conference on Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD 13 (Ashmun, 1995; Look, 1989).

Immunogen

Leukaemic cells from a case of chronic myeloid leukaemia (CML).

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Quality

Evaluated by flow cytometry using human PBMCs

Specificity

Antibody recognizes Human CD13 antigen.

Storage and Stability

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Target description

150 kDa (Turni, 2002; Ashmun, 1995; Look, 1989)